propidium iodide thermo fisher Search Results


propidium iodide (pi  (Fisher Scientific)
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Fisher Scientific propidium iodide (pi
Propidium Iodide (Pi, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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propidium iodide  (Fisher Scientific)
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Fisher Scientific propidium iodide
a Representative confocal laser scanning microscopic image of a root tip of an 8-day-old dph1-1 pDPH1:DPH1-GFP (line Y22-10) seedling stained with <t>propidium</t> iodide (PI, red). Scale bars, 50 μm. b Immunoblot probed with antibodies specific for detection of eEF2 lacking the diphthamide modification (unmodified), recognizing all forms of eEF2 (global), or serving as a loading control (anti-β-Actin for MCF-7, anti-plant ACTIN for A. thaliana ), respectively. Wild-type (WT) and DPH1 − / − mutant human cell line MCF-7 served as controls (left). c MS/MS spectra of eEF2 peptide 684-GICFEVCDVVLHSDAIHR-701 from WT (top) and dph1-1 mutant (bottom), with diphthamide ( D ) or without diphthamide ( N ) modification on H700 respectively ( C : carbamidomethylation). The selected monoisotopic precursor m / z ( z = +4) is given in each diagram; all detected y fragment ions are shown in blue. Red/pink lines visualize the consistent m / z difference of 71.055 between equivalent y n 2+ ions of the two genotypes. Tissues were shoots of 16-day-old 0.5× MS-grown seedlings ( b , c ). d Rates of ribosomal −1 frameshifting error. We normalized the ratio of firefly/renilla luciferase activity for a test −1 frameshift reporter construct to the ratio for an in-frame control reporter construct, as measured in transiently transfected Arabidopsis leaf mesophyll protoplasts prepared from 4-week-old soil-grown dph1 mutants and wild-type plants. Mean ± s.d., n = 3 independently transfected replicate aliquots of protoplasts, shown normalized to the wild-type (see Supplementary Fig. ). Significant differences from WT: ** P < 0.01 (one-way ANOVA, Tukey’s test).
Propidium Iodide, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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membrane-impermeable propidium iodide  (Fisher Scientific)
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Fisher Scientific membrane-impermeable propidium iodide
a Representative confocal laser scanning microscopic image of a root tip of an 8-day-old dph1-1 pDPH1:DPH1-GFP (line Y22-10) seedling stained with <t>propidium</t> iodide (PI, red). Scale bars, 50 μm. b Immunoblot probed with antibodies specific for detection of eEF2 lacking the diphthamide modification (unmodified), recognizing all forms of eEF2 (global), or serving as a loading control (anti-β-Actin for MCF-7, anti-plant ACTIN for A. thaliana ), respectively. Wild-type (WT) and DPH1 − / − mutant human cell line MCF-7 served as controls (left). c MS/MS spectra of eEF2 peptide 684-GICFEVCDVVLHSDAIHR-701 from WT (top) and dph1-1 mutant (bottom), with diphthamide ( D ) or without diphthamide ( N ) modification on H700 respectively ( C : carbamidomethylation). The selected monoisotopic precursor m / z ( z = +4) is given in each diagram; all detected y fragment ions are shown in blue. Red/pink lines visualize the consistent m / z difference of 71.055 between equivalent y n 2+ ions of the two genotypes. Tissues were shoots of 16-day-old 0.5× MS-grown seedlings ( b , c ). d Rates of ribosomal −1 frameshifting error. We normalized the ratio of firefly/renilla luciferase activity for a test −1 frameshift reporter construct to the ratio for an in-frame control reporter construct, as measured in transiently transfected Arabidopsis leaf mesophyll protoplasts prepared from 4-week-old soil-grown dph1 mutants and wild-type plants. Mean ± s.d., n = 3 independently transfected replicate aliquots of protoplasts, shown normalized to the wild-type (see Supplementary Fig. ). Significant differences from WT: ** P < 0.01 (one-way ANOVA, Tukey’s test).
Membrane Impermeable Propidium Iodide, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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propidium iodide 11599296  (Fisher Scientific)
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Fisher Scientific propidium iodide 11599296
a Representative confocal laser scanning microscopic image of a root tip of an 8-day-old dph1-1 pDPH1:DPH1-GFP (line Y22-10) seedling stained with <t>propidium</t> iodide (PI, red). Scale bars, 50 μm. b Immunoblot probed with antibodies specific for detection of eEF2 lacking the diphthamide modification (unmodified), recognizing all forms of eEF2 (global), or serving as a loading control (anti-β-Actin for MCF-7, anti-plant ACTIN for A. thaliana ), respectively. Wild-type (WT) and DPH1 − / − mutant human cell line MCF-7 served as controls (left). c MS/MS spectra of eEF2 peptide 684-GICFEVCDVVLHSDAIHR-701 from WT (top) and dph1-1 mutant (bottom), with diphthamide ( D ) or without diphthamide ( N ) modification on H700 respectively ( C : carbamidomethylation). The selected monoisotopic precursor m / z ( z = +4) is given in each diagram; all detected y fragment ions are shown in blue. Red/pink lines visualize the consistent m / z difference of 71.055 between equivalent y n 2+ ions of the two genotypes. Tissues were shoots of 16-day-old 0.5× MS-grown seedlings ( b , c ). d Rates of ribosomal −1 frameshifting error. We normalized the ratio of firefly/renilla luciferase activity for a test −1 frameshift reporter construct to the ratio for an in-frame control reporter construct, as measured in transiently transfected Arabidopsis leaf mesophyll protoplasts prepared from 4-week-old soil-grown dph1 mutants and wild-type plants. Mean ± s.d., n = 3 independently transfected replicate aliquots of protoplasts, shown normalized to the wild-type (see Supplementary Fig. ). Significant differences from WT: ** P < 0.01 (one-way ANOVA, Tukey’s test).
Propidium Iodide 11599296, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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4 μg/ml propidium iodide  (Fisher Scientific)
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Fisher Scientific 4 μg/ml propidium iodide
a Representative confocal laser scanning microscopic image of a root tip of an 8-day-old dph1-1 pDPH1:DPH1-GFP (line Y22-10) seedling stained with <t>propidium</t> iodide (PI, red). Scale bars, 50 μm. b Immunoblot probed with antibodies specific for detection of eEF2 lacking the diphthamide modification (unmodified), recognizing all forms of eEF2 (global), or serving as a loading control (anti-β-Actin for MCF-7, anti-plant ACTIN for A. thaliana ), respectively. Wild-type (WT) and DPH1 − / − mutant human cell line MCF-7 served as controls (left). c MS/MS spectra of eEF2 peptide 684-GICFEVCDVVLHSDAIHR-701 from WT (top) and dph1-1 mutant (bottom), with diphthamide ( D ) or without diphthamide ( N ) modification on H700 respectively ( C : carbamidomethylation). The selected monoisotopic precursor m / z ( z = +4) is given in each diagram; all detected y fragment ions are shown in blue. Red/pink lines visualize the consistent m / z difference of 71.055 between equivalent y n 2+ ions of the two genotypes. Tissues were shoots of 16-day-old 0.5× MS-grown seedlings ( b , c ). d Rates of ribosomal −1 frameshifting error. We normalized the ratio of firefly/renilla luciferase activity for a test −1 frameshift reporter construct to the ratio for an in-frame control reporter construct, as measured in transiently transfected Arabidopsis leaf mesophyll protoplasts prepared from 4-week-old soil-grown dph1 mutants and wild-type plants. Mean ± s.d., n = 3 independently transfected replicate aliquots of protoplasts, shown normalized to the wild-type (see Supplementary Fig. ). Significant differences from WT: ** P < 0.01 (one-way ANOVA, Tukey’s test).
4 μg/Ml Propidium Iodide, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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propidium iodide fisher scientific #00-6990-50  (Fisher Scientific)
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a Representative confocal laser scanning microscopic image of a root tip of an 8-day-old dph1-1 pDPH1:DPH1-GFP (line Y22-10) seedling stained with <t>propidium</t> iodide (PI, red). Scale bars, 50 μm. b Immunoblot probed with antibodies specific for detection of eEF2 lacking the diphthamide modification (unmodified), recognizing all forms of eEF2 (global), or serving as a loading control (anti-β-Actin for MCF-7, anti-plant ACTIN for A. thaliana ), respectively. Wild-type (WT) and DPH1 − / − mutant human cell line MCF-7 served as controls (left). c MS/MS spectra of eEF2 peptide 684-GICFEVCDVVLHSDAIHR-701 from WT (top) and dph1-1 mutant (bottom), with diphthamide ( D ) or without diphthamide ( N ) modification on H700 respectively ( C : carbamidomethylation). The selected monoisotopic precursor m / z ( z = +4) is given in each diagram; all detected y fragment ions are shown in blue. Red/pink lines visualize the consistent m / z difference of 71.055 between equivalent y n 2+ ions of the two genotypes. Tissues were shoots of 16-day-old 0.5× MS-grown seedlings ( b , c ). d Rates of ribosomal −1 frameshifting error. We normalized the ratio of firefly/renilla luciferase activity for a test −1 frameshift reporter construct to the ratio for an in-frame control reporter construct, as measured in transiently transfected Arabidopsis leaf mesophyll protoplasts prepared from 4-week-old soil-grown dph1 mutants and wild-type plants. Mean ± s.d., n = 3 independently transfected replicate aliquots of protoplasts, shown normalized to the wild-type (see Supplementary Fig. ). Significant differences from WT: ** P < 0.01 (one-way ANOVA, Tukey’s test).
Propidium Iodide Fisher Scientific #00 6990 50, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fungalight cfda, am/propidium iodide yeast vitality kit  (Fisher Scientific)
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Fisher Scientific fungalight cfda, am/propidium iodide yeast vitality kit
a Representative confocal laser scanning microscopic image of a root tip of an 8-day-old dph1-1 pDPH1:DPH1-GFP (line Y22-10) seedling stained with <t>propidium</t> iodide (PI, red). Scale bars, 50 μm. b Immunoblot probed with antibodies specific for detection of eEF2 lacking the diphthamide modification (unmodified), recognizing all forms of eEF2 (global), or serving as a loading control (anti-β-Actin for MCF-7, anti-plant ACTIN for A. thaliana ), respectively. Wild-type (WT) and DPH1 − / − mutant human cell line MCF-7 served as controls (left). c MS/MS spectra of eEF2 peptide 684-GICFEVCDVVLHSDAIHR-701 from WT (top) and dph1-1 mutant (bottom), with diphthamide ( D ) or without diphthamide ( N ) modification on H700 respectively ( C : carbamidomethylation). The selected monoisotopic precursor m / z ( z = +4) is given in each diagram; all detected y fragment ions are shown in blue. Red/pink lines visualize the consistent m / z difference of 71.055 between equivalent y n 2+ ions of the two genotypes. Tissues were shoots of 16-day-old 0.5× MS-grown seedlings ( b , c ). d Rates of ribosomal −1 frameshifting error. We normalized the ratio of firefly/renilla luciferase activity for a test −1 frameshift reporter construct to the ratio for an in-frame control reporter construct, as measured in transiently transfected Arabidopsis leaf mesophyll protoplasts prepared from 4-week-old soil-grown dph1 mutants and wild-type plants. Mean ± s.d., n = 3 independently transfected replicate aliquots of protoplasts, shown normalized to the wild-type (see Supplementary Fig. ). Significant differences from WT: ** P < 0.01 (one-way ANOVA, Tukey’s test).
Fungalight Cfda, Am/Propidium Iodide Yeast Vitality Kit, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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propidium iodide staining  (Fisher Scientific)
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Fisher Scientific propidium iodide staining
a Representative confocal laser scanning microscopic image of a root tip of an 8-day-old dph1-1 pDPH1:DPH1-GFP (line Y22-10) seedling stained with <t>propidium</t> iodide (PI, red). Scale bars, 50 μm. b Immunoblot probed with antibodies specific for detection of eEF2 lacking the diphthamide modification (unmodified), recognizing all forms of eEF2 (global), or serving as a loading control (anti-β-Actin for MCF-7, anti-plant ACTIN for A. thaliana ), respectively. Wild-type (WT) and DPH1 − / − mutant human cell line MCF-7 served as controls (left). c MS/MS spectra of eEF2 peptide 684-GICFEVCDVVLHSDAIHR-701 from WT (top) and dph1-1 mutant (bottom), with diphthamide ( D ) or without diphthamide ( N ) modification on H700 respectively ( C : carbamidomethylation). The selected monoisotopic precursor m / z ( z = +4) is given in each diagram; all detected y fragment ions are shown in blue. Red/pink lines visualize the consistent m / z difference of 71.055 between equivalent y n 2+ ions of the two genotypes. Tissues were shoots of 16-day-old 0.5× MS-grown seedlings ( b , c ). d Rates of ribosomal −1 frameshifting error. We normalized the ratio of firefly/renilla luciferase activity for a test −1 frameshift reporter construct to the ratio for an in-frame control reporter construct, as measured in transiently transfected Arabidopsis leaf mesophyll protoplasts prepared from 4-week-old soil-grown dph1 mutants and wild-type plants. Mean ± s.d., n = 3 independently transfected replicate aliquots of protoplasts, shown normalized to the wild-type (see Supplementary Fig. ). Significant differences from WT: ** P < 0.01 (one-way ANOVA, Tukey’s test).
Propidium Iodide Staining, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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propidium iodide fisher scientific  (Fisher Scientific)
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a Representative confocal laser scanning microscopic image of a root tip of an 8-day-old dph1-1 pDPH1:DPH1-GFP (line Y22-10) seedling stained with <t>propidium</t> iodide (PI, red). Scale bars, 50 μm. b Immunoblot probed with antibodies specific for detection of eEF2 lacking the diphthamide modification (unmodified), recognizing all forms of eEF2 (global), or serving as a loading control (anti-β-Actin for MCF-7, anti-plant ACTIN for A. thaliana ), respectively. Wild-type (WT) and DPH1 − / − mutant human cell line MCF-7 served as controls (left). c MS/MS spectra of eEF2 peptide 684-GICFEVCDVVLHSDAIHR-701 from WT (top) and dph1-1 mutant (bottom), with diphthamide ( D ) or without diphthamide ( N ) modification on H700 respectively ( C : carbamidomethylation). The selected monoisotopic precursor m / z ( z = +4) is given in each diagram; all detected y fragment ions are shown in blue. Red/pink lines visualize the consistent m / z difference of 71.055 between equivalent y n 2+ ions of the two genotypes. Tissues were shoots of 16-day-old 0.5× MS-grown seedlings ( b , c ). d Rates of ribosomal −1 frameshifting error. We normalized the ratio of firefly/renilla luciferase activity for a test −1 frameshift reporter construct to the ratio for an in-frame control reporter construct, as measured in transiently transfected Arabidopsis leaf mesophyll protoplasts prepared from 4-week-old soil-grown dph1 mutants and wild-type plants. Mean ± s.d., n = 3 independently transfected replicate aliquots of protoplasts, shown normalized to the wild-type (see Supplementary Fig. ). Significant differences from WT: ** P < 0.01 (one-way ANOVA, Tukey’s test).
Propidium Iodide Fisher Scientific, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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annexin-v fitc/propidium iodide (pi) double-staining method  (Fisher Scientific)
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Fisher Scientific annexin-v fitc/propidium iodide (pi) double-staining method
a Representative confocal laser scanning microscopic image of a root tip of an 8-day-old dph1-1 pDPH1:DPH1-GFP (line Y22-10) seedling stained with <t>propidium</t> iodide (PI, red). Scale bars, 50 μm. b Immunoblot probed with antibodies specific for detection of eEF2 lacking the diphthamide modification (unmodified), recognizing all forms of eEF2 (global), or serving as a loading control (anti-β-Actin for MCF-7, anti-plant ACTIN for A. thaliana ), respectively. Wild-type (WT) and DPH1 − / − mutant human cell line MCF-7 served as controls (left). c MS/MS spectra of eEF2 peptide 684-GICFEVCDVVLHSDAIHR-701 from WT (top) and dph1-1 mutant (bottom), with diphthamide ( D ) or without diphthamide ( N ) modification on H700 respectively ( C : carbamidomethylation). The selected monoisotopic precursor m / z ( z = +4) is given in each diagram; all detected y fragment ions are shown in blue. Red/pink lines visualize the consistent m / z difference of 71.055 between equivalent y n 2+ ions of the two genotypes. Tissues were shoots of 16-day-old 0.5× MS-grown seedlings ( b , c ). d Rates of ribosomal −1 frameshifting error. We normalized the ratio of firefly/renilla luciferase activity for a test −1 frameshift reporter construct to the ratio for an in-frame control reporter construct, as measured in transiently transfected Arabidopsis leaf mesophyll protoplasts prepared from 4-week-old soil-grown dph1 mutants and wild-type plants. Mean ± s.d., n = 3 independently transfected replicate aliquots of protoplasts, shown normalized to the wild-type (see Supplementary Fig. ). Significant differences from WT: ** P < 0.01 (one-way ANOVA, Tukey’s test).
Annexin V Fitc/Propidium Iodide (Pi) Double Staining Method, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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propidium iodide dye exclusion assay  (Fisher Scientific)
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a Representative confocal laser scanning microscopic image of a root tip of an 8-day-old dph1-1 pDPH1:DPH1-GFP (line Y22-10) seedling stained with <t>propidium</t> iodide (PI, red). Scale bars, 50 μm. b Immunoblot probed with antibodies specific for detection of eEF2 lacking the diphthamide modification (unmodified), recognizing all forms of eEF2 (global), or serving as a loading control (anti-β-Actin for MCF-7, anti-plant ACTIN for A. thaliana ), respectively. Wild-type (WT) and DPH1 − / − mutant human cell line MCF-7 served as controls (left). c MS/MS spectra of eEF2 peptide 684-GICFEVCDVVLHSDAIHR-701 from WT (top) and dph1-1 mutant (bottom), with diphthamide ( D ) or without diphthamide ( N ) modification on H700 respectively ( C : carbamidomethylation). The selected monoisotopic precursor m / z ( z = +4) is given in each diagram; all detected y fragment ions are shown in blue. Red/pink lines visualize the consistent m / z difference of 71.055 between equivalent y n 2+ ions of the two genotypes. Tissues were shoots of 16-day-old 0.5× MS-grown seedlings ( b , c ). d Rates of ribosomal −1 frameshifting error. We normalized the ratio of firefly/renilla luciferase activity for a test −1 frameshift reporter construct to the ratio for an in-frame control reporter construct, as measured in transiently transfected Arabidopsis leaf mesophyll protoplasts prepared from 4-week-old soil-grown dph1 mutants and wild-type plants. Mean ± s.d., n = 3 independently transfected replicate aliquots of protoplasts, shown normalized to the wild-type (see Supplementary Fig. ). Significant differences from WT: ** P < 0.01 (one-way ANOVA, Tukey’s test).
Propidium Iodide Dye Exclusion Assay, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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0.2% propidium iodide  (Fisher Scientific)
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a Representative confocal laser scanning microscopic image of a root tip of an 8-day-old dph1-1 pDPH1:DPH1-GFP (line Y22-10) seedling stained with <t>propidium</t> iodide (PI, red). Scale bars, 50 μm. b Immunoblot probed with antibodies specific for detection of eEF2 lacking the diphthamide modification (unmodified), recognizing all forms of eEF2 (global), or serving as a loading control (anti-β-Actin for MCF-7, anti-plant ACTIN for A. thaliana ), respectively. Wild-type (WT) and DPH1 − / − mutant human cell line MCF-7 served as controls (left). c MS/MS spectra of eEF2 peptide 684-GICFEVCDVVLHSDAIHR-701 from WT (top) and dph1-1 mutant (bottom), with diphthamide ( D ) or without diphthamide ( N ) modification on H700 respectively ( C : carbamidomethylation). The selected monoisotopic precursor m / z ( z = +4) is given in each diagram; all detected y fragment ions are shown in blue. Red/pink lines visualize the consistent m / z difference of 71.055 between equivalent y n 2+ ions of the two genotypes. Tissues were shoots of 16-day-old 0.5× MS-grown seedlings ( b , c ). d Rates of ribosomal −1 frameshifting error. We normalized the ratio of firefly/renilla luciferase activity for a test −1 frameshift reporter construct to the ratio for an in-frame control reporter construct, as measured in transiently transfected Arabidopsis leaf mesophyll protoplasts prepared from 4-week-old soil-grown dph1 mutants and wild-type plants. Mean ± s.d., n = 3 independently transfected replicate aliquots of protoplasts, shown normalized to the wild-type (see Supplementary Fig. ). Significant differences from WT: ** P < 0.01 (one-way ANOVA, Tukey’s test).
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Image Search Results


a Representative confocal laser scanning microscopic image of a root tip of an 8-day-old dph1-1 pDPH1:DPH1-GFP (line Y22-10) seedling stained with propidium iodide (PI, red). Scale bars, 50 μm. b Immunoblot probed with antibodies specific for detection of eEF2 lacking the diphthamide modification (unmodified), recognizing all forms of eEF2 (global), or serving as a loading control (anti-β-Actin for MCF-7, anti-plant ACTIN for A. thaliana ), respectively. Wild-type (WT) and DPH1 − / − mutant human cell line MCF-7 served as controls (left). c MS/MS spectra of eEF2 peptide 684-GICFEVCDVVLHSDAIHR-701 from WT (top) and dph1-1 mutant (bottom), with diphthamide ( D ) or without diphthamide ( N ) modification on H700 respectively ( C : carbamidomethylation). The selected monoisotopic precursor m / z ( z = +4) is given in each diagram; all detected y fragment ions are shown in blue. Red/pink lines visualize the consistent m / z difference of 71.055 between equivalent y n 2+ ions of the two genotypes. Tissues were shoots of 16-day-old 0.5× MS-grown seedlings ( b , c ). d Rates of ribosomal −1 frameshifting error. We normalized the ratio of firefly/renilla luciferase activity for a test −1 frameshift reporter construct to the ratio for an in-frame control reporter construct, as measured in transiently transfected Arabidopsis leaf mesophyll protoplasts prepared from 4-week-old soil-grown dph1 mutants and wild-type plants. Mean ± s.d., n = 3 independently transfected replicate aliquots of protoplasts, shown normalized to the wild-type (see Supplementary Fig. ). Significant differences from WT: ** P < 0.01 (one-way ANOVA, Tukey’s test).

Journal: Nature Communications

Article Title: Translational fidelity and growth of Arabidopsis require stress-sensitive diphthamide biosynthesis

doi: 10.1038/s41467-022-31712-7

Figure Lengend Snippet: a Representative confocal laser scanning microscopic image of a root tip of an 8-day-old dph1-1 pDPH1:DPH1-GFP (line Y22-10) seedling stained with propidium iodide (PI, red). Scale bars, 50 μm. b Immunoblot probed with antibodies specific for detection of eEF2 lacking the diphthamide modification (unmodified), recognizing all forms of eEF2 (global), or serving as a loading control (anti-β-Actin for MCF-7, anti-plant ACTIN for A. thaliana ), respectively. Wild-type (WT) and DPH1 − / − mutant human cell line MCF-7 served as controls (left). c MS/MS spectra of eEF2 peptide 684-GICFEVCDVVLHSDAIHR-701 from WT (top) and dph1-1 mutant (bottom), with diphthamide ( D ) or without diphthamide ( N ) modification on H700 respectively ( C : carbamidomethylation). The selected monoisotopic precursor m / z ( z = +4) is given in each diagram; all detected y fragment ions are shown in blue. Red/pink lines visualize the consistent m / z difference of 71.055 between equivalent y n 2+ ions of the two genotypes. Tissues were shoots of 16-day-old 0.5× MS-grown seedlings ( b , c ). d Rates of ribosomal −1 frameshifting error. We normalized the ratio of firefly/renilla luciferase activity for a test −1 frameshift reporter construct to the ratio for an in-frame control reporter construct, as measured in transiently transfected Arabidopsis leaf mesophyll protoplasts prepared from 4-week-old soil-grown dph1 mutants and wild-type plants. Mean ± s.d., n = 3 independently transfected replicate aliquots of protoplasts, shown normalized to the wild-type (see Supplementary Fig. ). Significant differences from WT: ** P < 0.01 (one-way ANOVA, Tukey’s test).

Article Snippet: Nuclear DNA was stained in 0.1 mg ml −1 propidium iodide while RNA was removed by 0.1 mg ml −1 RNase A (Fisher Scientific).

Techniques: Staining, Western Blot, Modification, Control, Mutagenesis, Tandem Mass Spectroscopy, Luciferase, Activity Assay, Construct, Transfection